mouse anti rat intercellular cell adhesion molecule 1 icam 1  (Bio-Rad)

Journal:

Article Title: Interactions between Brain Endothelial Cells and Human T-Cell Leukemia Virus Type 1-Infected Lymphocytes: Mechanisms of Viral Entry into the Central Nervous System

doi:

Figure Lengend Snippet: HTLV-1-infected lymphocyte adhesion to and migration through brain endothelial cells. (A) Adhesion and transendothelial migration of HTLV-1-infected and control lymphocytes through GPNT cell monolayers. Each point represents the mean obtained from a minimum of six separate wells or three separate filters from one experiment representative of three. Results are expressed as the fractional T-lymphocyte adhesion to or migration through endothelial cells. (B) Expression of LFA-1 and VLA-4 by HTLV-1-infected and control lymphocytes using FACScan analysis. (C) ELISA determination of ICAM-1 and VCAM-1 expression by GPNT cells following stimulation with 100 U of TNF-α and IL-1β per ml for 24 h. Results are from six separate wells from one representative experiment. O.D., optical density. (D) Effect of monoclonal anti-LFA-1 and anti-VLA-4 antibodies on lymphocyte adhesion to GPNT cells. The concentrations of antibodies used are those indicated by the manufacturer as inhibiting the adhesion of different cell types (10 μg/ml). Results are for six separate wells from one representative experiment.

Article Snippet: Mouse anti-rat intercellular cell adhesion molecule 1 (ICAM-1) (1A29) monoclonal antibody was obtained from Serotec (Oxford, United Kingdom).

Techniques: Infection, Migration, Expressing, Enzyme-linked Immunosorbent Assay